Reading ZymoBIOMICS fasta files in get.groups

Commands in mothur
1

Greetings!

I am currently trying to run the get.groups command. I have recieved the fasta files from ZymoBIOMICS that were used when my samples were sequenced. I tried adding the word ‘Mock’ before each fasta file. I am trying get the current command to run:

get.groups(count=stability.trim.contigs.good.unique.good.filter.unique.precluster.denovo.vsearch.pick.pick.count_table, fasta=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.fasta, groups=Mock)

When I do this, I receive this message:

Your file does NOT contain sequences from the groups you wish to get.

Selected 0 sequences from your count file.

Your file does NOT contain sequences from the groups you wish to get.

Selected 0 sequences from your fasta file.

There are 12 fasta files included for the 16S/18S. How do I incorporate these into my code?

2

If you run count.groups(count=stability.trim.contigs.good.unique.good.filter.unique.precluster.denovo.vsearch.pick.pick.count_table) what do you get? You should see the name of your mock community listed in the output

Pat

3

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