Hi,
I am trying to do the read.otu command with a data set of 517 sequences. I repeatedly get errors related to my group file. The latest is " :Error: Sequence ‘16s20M6-08D03533FH05.ab1’ was not found in the group file, please correct.", yet when I open my groups file and look at it, the sequence name is there, just like all the others. I have had this error before, and still have not figured out how to get beyond this step. I created my group file in excel, using the sequence names found in my good.filter.fasta file, and saved it as a tab delimited text file. I think all the names match up between my fasta file and my groups file. Any suggestions? Thanks. Lorraine Olendzenski