I have an additional difficulty, I have both the 32 bit Windows GUI and the 64 bit Linux GUI installed. Both work fine until I try using chimera.uchime to detect chimeras. I have tried both checking “self” as the refset and using the silva.gold refence suggested in the Manual/Analysis Examples. In both instances, the program launches but then stops working. Basically it does not finish the job.
The Windows system: Windows 8, 64 bit, running the 32-bit mothurWinGUI, 6 Gb RAM
The Linux system: Ubuntu 12.10, 64 bit, running the 64-bit mothurGUI, 8 Gb RAM
here is the last entry in the logfile:
mothur > chimera.uchime(fasta=Beckerpr.unique.good.filter.unique.precluster.fasta, reference=/home/ansilab/Programs/mothurGUI/mothur/ref/silva.gold.ng.fasta, chimealns=T, dereplicate=T, processors=2)
Using 2 processors.
uchime by Robert C. Edgar
UCLUST downloads
This code is donated to the public domain.
Checking sequences from Beckerpr.unique.good.filter.unique.precluster.fasta …
And here is the last entry on the Output Screen:
Current working directory: /home/ansilab/Programs/mothurGUI/
[H[2J
mothur > chimera.uchime(fasta=Beckerpr.unique.good.filter.unique.precluster.fasta, reference=/home/ansilab/Programs/mothurGUI/mothur/ref/silva.gold.ng.fasta, chimealns=T, dereplicate=T, processors=2)
Using 2 processors.
uchime by Robert C. Edgar
UCLUST downloads
This code is donated to the public domain.
Checking sequences from Beckerpr.unique.good.filter.unique.precluster.fasta …
uchime v4.2.40
by Robert C. Edgar
UCLUST downloads
This code is donated to the public domain.
uchime v4.2.40
by Robert C. Edgar
UCLUST downloads
This code is donated to the public domain.
After that it just stops without providing any output.
In addition, for the Linux 64-bit mothurGUI, everytime it tries to find/write the current files to the screen/logfile it gives the following error(s) on screen. Not sure which refers to what:
Exited with error code 0
Exited with error code -1
and this is what is present in the output window:
Current working directory: /home/ansilab/Programs/mothurGUI/
[H[2J
mothur > chimera.uchime(fasta=Beckerpr.unique.good.filter.unique.precluster.fasta, reference=/home/ansilab/Programs/mothurGUI/mothur/ref/silva.gold.ng.fasta, chimealns=T, dereplicate=T, processors=2)