Make.sra command

Announcements
1

Dear all, sorry to interrupt.

Can anyone help me with the Make.sra command. I have been trying since morning but it’s night now no output.

Some command I used:

mothur > make.sra(sff=AP.sff, oligos=AP.txt, pdiffs=2, bdiffs=1, project=test.project, mimark=MIMarksData.txt)

/******************************************/
Running command: sffinfo(sff=C:\mothur\AP.sff, oligos=C:\mothur\AP.txt, pdiffs=2, bdiffs=1, tdiffs=3)
Extracting info from C:\mothur\AP.sff …
10000
20000
30000
40000
50000
60000
70000
80000
90000
100000
110000
119813
It took 483 secs to extract 119813.

Output File Names:
C:\mothur\AP.Treatment.24.1_AACGTATC.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Control.24.1_AACGTATG.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Treatment.48.1_AACGTATT.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Control.48.1_AACGTCAC.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Treatment.48.2_CAGTCATT.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Control.R.0_CATATGGT.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Treatment.24.3_TAATTGGA.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Control.0_TCCGTCAT.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Control.48.3_TCTACGTT.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Treatment.24.2_TCTCTTCT.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Control.48.2_TGGCGAGA.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Control.24.3_TGGTAAGT.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Treatment.48.3_TTACCAAT.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.Control.24.2_TTCAGGCT.GAGTTTGATCCTGGCTCA.sff
C:\mothur\AP.fasta
C:\mothur\AP.qual
C:\mothur\AP.flow
C:\mothur\AP.scrap.sff

/******************************************/
[ERROR]: MIMarks file is missing group Control.0_TCCGTCAT.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Control.24.1_AACGTATG.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Control.24.2_TTCAGGCT.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Control.24.3_TGGTAAGT.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Control.48.1_AACGTCAC.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Control.48.2_TGGCGAGA.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Control.48.3_TCTACGTT.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Control.R.0_CATATGGT.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Treatment.24.1_AACGTATC.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Treatment.24.2_TCTCTTCT.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Treatment.24.3_TAATTGGA.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Treatment.48.1_AACGTATT.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Treatment.48.2_CAGTCATT.GAGTTTGATCCTGGCTCA, please correct.
[ERROR]: MIMarks file is missing group Treatment.48.3_TTACCAAT.GAGTTTGATCCTGGCTCA, please correct.

2

Actually, there is no output in the folder.

3

The command in the released version of mothur is not ready for prime time. The next release should have our first working version of the function.

4

Dear Dr. Pat, thanks a lot for your clarification. I will wait for the next release.