I’ve been running problems using make.sra command. It used to work great.
Now I have issues like make.sra only generates submission.xml and submit.ready files, without sequences for each samples.
I used raw sequencing data, make.sra (file=xx.file, project=xx.project, mimark=xx.txt)
my xx.file contains information
name_1 DMP04993_L1_name_1_1.fq.gz DMP04993_L1_name_1_2.fq.gz
name_2 DMP04993_L1_name_2_1.fq.gz DMP04993_L1_name_2_2.fq.gz
If I run make.sra(fastq=xx.fastq, oligos=xx.oligos, project=xx.project, mimark=xx.txt)
the fastq file is made by merging all the raw data(forward and reverse fastq) files together.
All the seqs end up in the scarp file.
How can I correct it, please?
Thanks you so much for your help