make.contigs--Segmentation fault: 11

mothur bugs
1

Hi there,

I am getting the following error when trying to run the make.contigs command. The previous files (1-17) aren’t having any issues. Not sure what to do… --Micah

mothur > make.contigs(file=hm.paired.files)

Processing file pair 7_S172_Sept_18_L001_R1_001.fastq - 7_S182_Sept_18_L001_R1_001.fastq (files 18 of 18) <<<<<
Making contigs…
M01479_272_000000000-C97K2_1_1102_16333_1373 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.
M01479_272_000000000-C97K2_1_1104_13608_19089 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.
M01479_272_000000000-C97K2_1_1107_3418_10751 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.
M01479_272_000000000-C97K2_1_1112_13646_26704 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.
M01479_272_000000000-C97K2_1_2102_10003_16258 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.
M01479_272_000000000-C97K2_1_2103_6667_25980 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.
M01479_272_000000000-C97K2_1_2107_12854_11036 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.
M01479_272_000000000-C97K2_1_2112_6123_20237 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.
Segmentation fault: 11

2

“Processing file pair 7_S172_Sept_18_L001_R1_001.fastq - 7_S182_Sept_18_L001_R1_001.fastq”

These both appear to be forward files from different samples based on the file names. Could this be a typo in the file file?

7_S172_Sept_18_L001_R1_001.fastq
7_S182_Sept_18_L001_R1_001.fastq

3

That could very well be… will check it out–thank you!

4

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