I wonder – can I use mothur to assemble forward and reverse 16S sequences?
Sequences generated by 454, starting from primer 63f and 518r. There are six samples.
Three forward and three reverse. Can I pool everything and make mothur provide me with contigs?
Ideally, this will indicate similarity (sequence overlap) and yield more robust taxonomy.
Comments are appreciated.
Sigmund
Not for 454 because the reads are not paired - i.e. the same DNA fragment isn’t getting sequenced in both directions.