I want to make a biome table and I have sample information as follows in a file called “design.table”:
SampleID Animal# T1 T2 Tissue
Samp_1 Def-1 PCD14/21 PBCD4 Duodenum
Samp_2 Def-1 PCD14/21 PBCD4 Jejunum
Samp_3 Def-1 PCD14/21 PBCD4 Ileum
Samp_4 Def-1 PCD14/21 PBCD4 Colon
Samp_5 Def-2 PCD14/21 PBCD4 Duodenum
Samp_6 Def-2 PCD14/21 PBCD4 Jejunum
When I tried to use this with “make.biom” command with “metadata = design.table”, it gives the following error:
[ERROR]: expected 4 items of data for sample Samp_17 read 5, quitting.
[ERROR]: expected 4 items of data for sample Samp_18 read 5, quitting.
[ERROR]: expected 4 items of data for sample Samp_19 read 5, quitting.
[ERROR]: expected 4 items of data for sample Samp_20 read 5, quitting.
[ERROR]: expected 4 items of data for sample Samp_21 read 5, quitting.
When I tried with just one column, it worked fine. Does this mean I can only have one additional column with samples info?