Hi,
I am facing an issue while running screen.seqs command on one of my datasets. After the command gets executed, I get a list that says some sequences are missing from my count file. But to my wonder, those sequences are present in my count file. The log reports are as below(Is there any common email id that can be used to send the log report and the count table if required?).
mothur > screen.seqs(fasta=sample1_150bp_final_header.unique.align, count=sample1_150bp_final_header.count_table, summary=sample1_150bp_final_header.unique.summary, start=6333, end=13862, maxhomop=8)
Using 1 processors.
Your count file does not include the sequence Sample1_100000 please correct.
Your count file does not include the sequence Sample1_100017 please correct.
Your count file does not include the sequence Sample1_100027 please correct.
Your count file does not include the sequence Sample1_100042 please correct.
Your count file does not include the sequence Sample1_10005 please correct.
Your count file does not include the sequence Sample1_100053 please correct.
Your count file does not include the sequence Sample1_100058 please correct.
Your count file does not include the sequence Sample1_100063 please correct.
Your count file does not include the sequence Sample1_100069 please correct.
Your count file does not include the sequence Sample1_100076 please correct.
Your count file does not include the sequence Sample1_1001 please correct.
Your count file does not include the sequence Sample1_10011 please correct.
And my count table says
Sample1_100000 2
Sample1_100017 1
Sample1_100020 1
Sample1_100022 3
Sample1_100024 1
Sample1_100027 1
Sample1_100028 5
Sample1_100029 1
Sample1_100030 1
Sample1_100032 1
Sample1_100033 1
Sample1_100034 1
Sample1_100035 1
Sample1_100036 1
Sample1_100039 1
Sample1_100040 3
Sample1_100041 1
Sample1_100042 1
I was just following the instructions in the miseq SOP. Kindly advise me on what needs to be done here.