Dear Mothur community,
Using the mothur version 1.40.5 I got a problem with the screen.seqs command. After make.contigs is successfully finished,
Start End NBases Ambigs Polymer NumSeqs
Minimum: 1 212 212 0 3 1
2.5%-tile: 1 421 421 0 4 4721
25%-tile: 1 446 446 0 4 47205
Median: 1 446 446 0 5 94410
75%-tile: 1 446 446 2 6 141615
97.5%-tile: 1 447 447 16 6 184099
Maximum: 1 583 583 43 211 188819
Mean: 1 443 443 2 5
of Seqs: 188819
It took 4 secs to summarize 188819 sequences.
Output File Names:
Mock.trim.contigs.summary
I continued with the screen.seqs command:
(mothur > screen.seqs(fasta=Mock.trim.contigs.fasta, group=Mock.contigs.groups, contigsreport=Mock.contigs.report, maxambig=0, minlength=446, maxlength=447, minoverlap=50).
Screen.seqs generated an ever-growing file (Mock.contigs.good.contigs). So I had to cancel the command after several GByte. Could anybody help?
Many thanks for your support,
Kind regards,
Goetz