Hello everybody!
I´m running analysis of my illumina sequences for the first time. Everything was going fine til the get.oturep- it gives me error: “Could not open total.final.dist.sorted.dist.temp”. I´m running it on a computer that has 24 processors and 64Gb RAM. The comand was:
mothur > get.oturep(column=total.final.dist, name=total.final.names, fasta=total.final.fasta, list=total.final.an.list, label=0.03,cutoff=0.15, large=true)
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Formatting matrix: |||||||||||||||||||||||||||||||[ERROR]: Could not open total.final.dist.sorted.dist.temp
Any idea? I guess I could try doing it with method=abundance in order to skip this problem, but I would like to know what happened.
Thank you,
Ana