I’m a new in 16S amplicon sequencing and I can not figure out how to convert or generate data from mothur into Calypso format in the manner of each OTU\taxonomic group has a count of sequences.
Could somebody provide a basic pipeline to do it or clarify my view of this situation. If it lead to the parsing of several output files it will not be a problem for me.
I was trying to obtain these data via biom.info(biom=tx.1.biom, label=0.03, printlevel=4, output=simple), but I receive only OUTs number per taxonomic group instead of the sequence number.
http://cgenome.net:8080/html/wiki/index.php/Calypso_Help (Demo project data matrix)
Thank you in advane!