Hello!
I’m having 2 different problems with 2 analyses:
In the first analysis, I can’t complete the chimera.uhime command. The accnos file was not complete and the error message below was exhibited:
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_7359_15838 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_14555_15870 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_27464_16008 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_20966_16078 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_22987_16117 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_15596_16123 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_26544_16416 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_4317_16619 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_12838_16623 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_23959_16653 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_16320_17060 with a total=0. Please correct.
[ERROR]: Your count table contains a sequence named M01967_11_000000000-A7L66_1_2113_9803_17066 with a total=0. Please correct.
What can I do?
The second problem is in other file and other analysis. I have to merge files in order to comparing two microbiomes. So, I merged the taxonomy, count_table and fasta files from both microbiomes, which were generated after the remove.lineage command. However, when I performed the next step which was the cluster.split command, it was started, but in few minutes was interrupted. The error message said that the fasta file used is not unique…
Could someone help me?
Thanks a lot!!!