Hi, I am a newbie to mothur.
I am trying to replicate the Costello stool analysis example http://www.mothur.org/wiki/Costello_stool_analysis using mothur v 1.17.3 in suse linux.
It shows the following error.
mothur> align.seqs(candidate=stool.trim.unique.fasta, template=silva.bacteria/silva.bacteria.fasta, processors=2)
Reading in the silva.bacteria/silva.bacteria.fasta template sequences... DONE.
Aligning sequences from stool.trim.unique.fasta ...
100
100
200
[ERROR]: stool.trim.unique.align11024.num.temp is blank. Please correct.
[ERROR]: stool.trim.unique.align11025.num.temp is blank. Please correct.
It took 103 secs to align 0 sequences.
I looked for the spaces, but couldn’t find and moreover its running fine with the same files in the windows system.
I would appreciate any information about the error and its cause?