mothur

All seem to run well, but then stability.trim.contigs.good.fasta is 0 KB?


#1

make.contigs was successful, generating 2 large files stability.trim.contigs.fasta (1,900,000 kb) and stability.trim.contigs.qual (5,113,816 kb), although the other 3 files stability.contigs.report, stability.scrap.contigs.qual, and stability.scap.contigs.fasta are all 0 kb.

I moved on anyway, and was halted after the count.seqs step, because stability.trim.contigs.good.fasta has 0 kb.

I had thought it was my file names (stability.file). Tried changing that a couple times, but the same problem keeps coming up.

Really confused and stuck. Please help. Thank you!


#2

I’ve been given some space on a server. Is it possible that this problem is caused by low disk space?


#3

The problem is probably from the parameters you used in screen.seqs. Can you post the output printed to the screen from running summary.seqs before and after running screen.seqs?

Pat