Make.contigs error :The number of sequences in the fasta file does not match the group/count_table file

Specific problem : It shows that I spliced 354959 sequences in total, but the summary.seqs of fasta file shows 570747 sequences. I do not know why this situation occues

Group count: 
Group_0	46079
Group_1	40237
Group_2	44860
Group_3	72493
Group_4	48151
Group_5	103139

Total of all groups is 354959

It took 27 secs to process 354959 sequences.

Output File Names: 
stability.trim.contigs.fasta
stability.scrap.contigs.fasta
stability.contigs_report
stability.contigs.count_table


mothur > summary.seqs(fasta=stability.trim.contigs.fasta)

Using 64 processors.

		Start	End	NBases	Ambigs	Polymer	NumSeqs
Minimum:	1	0	0	0	1	1
2.5%-tile:	1	345	345	0	4	14269
25%-tile:	1	406	406	0	4	142687
Median: 	1	408	408	0	5	285374
75%-tile:	1	409	409	0	5	428061
97.5%-tile:	1	427	427	4	6	556479
Maximum:	1	890	888	67	211	570747
Mean:	1	406	405	0	4
# of Seqs:	570747

It took 2 secs to summarize 570747 sequences.

Output File Names:
stability.trim.contigs.summary

Can you try rerunning both make.contigs and summary.seqs with processors=6? I wonder if something weird is happening because you are using so many processors

Pat

I set processor to 6 and 8, respectively, but both failed, however, because the script was committed and there were no errors when running a command line in the window. I don’t know why

Can you post the commands and error messages you are getting? I’m not sure what you mean by it “failing” or “splicing”. I wonder if you’re possibly analyzing the same data by different appraoches simultaneously which could screw things up

Pat