Any news on when the new version will be released with updated trim.flows/shhh.flows command that can deal with acyclic flows? Sorry, don’t mean to pester. The Mothur team is always fantastic with updates, troubleshooting help, and making things uber-user friendly. Hence, feel terrible being impatient like this, but…
It will likely be Monday. But don’t expect any miracles with flow pattern B using the current 454 image processing software. With amplicons it’s pretty lousy compared to flow pattern A (and IonTorrent is just dreadful). Hopefully 454’s stuff will be fixed in the next release.