uchime error?

mothur bugs
1

Hi there,

I’ve been running into a problem with uchime sometimes on version 1.38.1. I get this error message for some of my samples and others not:

[Error]: Could not open stability.trim.contigs.good.unique.good.filter.unique.denovo.uchime.chimeras…

Using this command: mothur > chimera.uchime(fasta=stability.trim.contigs.good.unique.good.filter.unique.fasta, count=stability.trim.contigs.good.unique.good.filter.count_table, dereplicate=t, processors=10).

Any ideas what’s going on here? Or should I worry about this error?

Thanks,

Jake

2

Okay, so we figured this error message out. We were trying to run too many assembled sequences (~6.3 million) through uchime and it crashed it on our small linux box (only 10 cores and 32 GB RAM). So problem was resolved by using our larger machine.