I am following the MiSeqSOP for the data analysis, but at the pre.cluster step, programme is stopping by itself. I have tried so many times and I am stuck.
Can you tell us what version of mothur you are running? What operating system are you using? What region are you sequencing? What chemistry are you using? What does it mean that it’s “stopping by itself”? Does the screen sit and not move or does the terminal close/give a warning?
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