Dear all,
I received 16S Amplicon raw data (containing adapters and primers) from the sequencing company. The sequences for the adapters and primers:
Bacterial 16S rRNA gene of the selected regions (16S V3-V4) were amplified using locus-specific
sequence primers with overhang adapters as below:
Forward overhang: 5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG‐[locus‐ specific sequence]
Reverse overhang: 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG‐[locus‐ specific sequence]
Bacterial 16S V3-V4 (5’ to 3’)
16S V3-V4 Forward: CCTACGGGNGGCWGCAG
16S V3-V4 Reverse: GACTACHVGGGTATCTAATCC
I’ve read the tutorial but am still confused on how to properly create the oligos files and to properly trim the sequences.