trim.flows for flow values of 1600?

The SOP tells us trim.flows by defalt trims flow file to the length of 450,which is for the titanium data. What if the total number of flow values in my file is 1600 ? The defalt will still be OK?

If you don’t mock community data from these runs, then I would stick with 450. If you do have mock community data, then you could increase that and see what effect there is on the error rate. Reports I’ve received from people is that the longer reads aren’t much better than the previous Titanium chemistry with 800 total flows.