Hi everyone, I am new in mothur so I have a question from my raw data:
I going to start to analyse my two fastq files (forward and reverse) with the reads of a miseq run of a mix of close to 300 experimental samples, with every sample having a different tag barcode in the forward and reverse primer to assign to their respective experiment. So my question is how I can classify my sequences to the proper experiment (group)? I was thinking in using in the make.contings command, the oligos options with the table of every tag to which experiment belongs, but I don’t know if in the new file will have attached for all the sequences its respective group, so I can use that for the next analyses steps.
Thank you for the help,