shhh.flows error at "Clustering flowgrams..."

mothur bugs
1

I am trying to use shhh.flows command in Obuntu

However, the process stop at the same point like…

.
.
.
39200 1428 1427.91
39300 1436 1435.55
39400 1444 1443.42
39500 1452 1451.36
39600 1459 1458.84
39700 1467 1466.34
39752 1470 1470.27

Total time: 1470 1470.27

Clustering flowgrams…
********************###########
Reading matrix: ||||||||||||||||||||||||||||||||||||||||||||||||||||

and then Mothur quit to terminal command line.

Processing other small size file is fine but I can’t do shhh.flows with this big size flow file.
What is the problem here?

Anyway, thank you for this great program.

2

This generally happens because you’ve exhausted the RAM in your computer. This can occur for several reasons. What is the command that you are giving to trim.flows? How many samples are there in your oligos file?

Pat