I am busy working on a sequenced dataset that targeted the ITS1 region, using primers ITS1-F and ITS2-R. I just finished with my make.contigs (screenshot attached). My question here is pertaining to the minlength parameter for the screen.seqs function. Previously, I have used 250 while working with the ITS2 target region. Would it be appropriate to use 250 as the minlenght in screen.seqs for the ITS1 target region as well?
Thanks in advance!