Hi, I was processing my miseq sequencing data of archaeal 16S rRNA genes. Thngs went well until I came to screen.seqs after alignment with silva database, like this
"Your groupfile does not include the sequence M03388_21_000000000-AUCY0M03388_21_000000000-AUCY0_1_2102_16350_19661 please correct.
Your groupfile does not include the sequence M03388_21_000000000-AUCY0_1_1104_5073_M03388_21_000000000-AUCY0_1_1104_5073_17696 please correct.
Your groupfile does not include the sequence M03388_21_000000000-AUCY0_1_1113_16930_14M03388_21_000000000-AUCY0_1_1113_16930_14783 please correct.
Your groupfile does not include the sequence M03388_21_000000000-AUCY0_1_1116M03388_21_000000000-AUCY0_1_1116_15369_19125 please correct.
Your groupfile does not include the sequence M03388_21_000000000-AUCY0_1_11M03388_21_000000000-AUCY0_1_1104_25215_9543 please correct.
Your groupfile does not include the sequence M03388_21_000000000-AUCY0_1_2106_24675_23208M03388_21_000000000-AUCY0_1_2106_24675_23208 please correct.
Your groupfile does not include the sequence M03388_21_000000000-AUCY0_1_211M03388_21_000000000-AUCY0_1_2110_6055_20301 please correct.
Your groupfile does not include the sequence M03388_21_000000000-M03388_21_000000000-AUCY0_1_1112_12906_1827 please correct.
Your groupfile does not include the sequence M03388_21_00000M03388_21_000000000-AUCY0_1_2119_19399_24542 please correct.
Your groupfile does not include the sequence M03388_21_0M03388_21_000000000-AUCY0_1_1101_11297_3998 please correct.
Your groupfile does not include the sequence M03M03388_21_000000000-AUCY0_1_1111_24106_8076 please correct.
Your groupfile does not include the sequence ambig please correct."
The original groupfile looks fine, anyone can help?