Just looking for some advice.
I have 32GB of RAM running on windows 10 (64 bit) with mothur 1.44.3.
I’m running 266 fastq files totaling 5GB in data so I don’t have much of a feel for how much RAM this should need but I’m having an issue at the cluster stage of my analysis after dist.seqs, following the MiSeq SOP with my RAM running out.
The logfile suggests mothur gets to around 31GB about 12-18 hours in and crashes (I imagine windows is using the rest of the RAM). I tried to use 1 processor instead of 12 after seeing that recommended, which started running at 4GB but then rapidly escalated to 31GB again and crashed like before. Does anyone have any suggestions on how to fix this?
Many thanks in advance!