Hi. i have a question while i’m using the lefse command.
I analyzed my samples from 2 time points(0 time and 1 month).
I got the p-value 0.013 using amova.
As i wanted to know which differences were between 2 time points, i used metastats then lefse.
I knew which OTUs were significantly different between 2 time points form metastats.
But during using lefse, it was said “You did not provide a class”.
I wonder the OTUs significantly different are in which time point and i want to know how i can use the class option and whether i should add the class in which file (design file or metadata file)