Pre.cluster command in mothur v.1.41.1: missmatched files?

mothur bugs
1

Hi There,

I am running the pre.cluster command in mothur v.1.41.1. I noticed that the screen output looks like some sequences are mixed up (see example for two files below):

Processing group G3_DO18_515564:
C3_DO18_515568 2200 25289 19331
G3_DO18_515564 0 35757 389
C3_DO18_515568 2300 25191 19429
C3_DO18_515568 2400 25086 19534
G3_DO18_515564 100 28242 7904

Processing group C5_DO18_515585:
C5_DO18_515585 0 31147 512
G4_DO18_515570 1300 24153 21078
G4_DO18_515570 1400 23969 21262
C5_DO18_515585 100 23239 8420
G4_DO18_515570 1500 23783 21448
C5_DO18_515585 200 21578 10081
G4_DO18_515570 1600 23641 21590
G4_DO18_515570 1700 23537 21694
C5_DO18_515585 300 20399 11260
G4_DO18_515570 1800 23430 21801
G4_DO18_515570 1900 23299 21932
C5_DO18_515585 400 19614 12045
G4_DO18_515570 2000 23221 22010
G4_DO18_515570 2100 23142 22089
C5_DO18_515585 500 18983 12676

Thoughts?

Thanks

2

It’s probably nothing - are you using multiple processors? If so, the data from each processor is coming to the screen unsorted. Everything that is written to the files should be fine…

Pat

3

I am using multiple processors but its not something i have ever noticed happening before (perhaps i never looked hard enough!).

Thanks for the info.

4

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