Pre.cluster bug

Commands in mothur
1

I get an error message when using the chimera.vsearch command, which points to specific sequences that either have a value of zero or are absent in the count_table file generated from the pre.cluster command I used in the previous step. I have re-run the pre.cluster command with different diff values (1,2,3 …) but to no avail.
May I know why I am getting this error message in the chimera.vsearch step?

PS: I do not run the filter.seqs command. Is this a possible reason?

2

We fixed this bug in our latest version. Can you give it a try? https://github.com/mothur/mothur/releases/tag/v1.41.2

3

I am pretty sure I used this version of the software. Nevertheless, let me try again. Thank you Sarah! I will keep you posted.

4

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