Hello,
I am having the following error, after removing chimeric sequences after vsearch command. The chimera.vsearch has not finished due to
[ERROR]: 700463F_697_H7K7TBCX3_2_1101_10000_12021 is not in your count table. Please correct.
mothur >
pre.cluster(fasta=NorpaBac.trim.contigs.good.unique.good.filter.unique.fasta, count=NorpaBac.trim.contigs.good.unique.good.filter.count_table, diffs=2)
mothur >
chimera.vsearch(fasta=NorpaBac.trim.contigs.good.unique.good.filter.unique.precluster.fasta, count=NorpaBac.trim.contigs.good.unique.good.filter.unique.precluster.count_table, dereplicate=t)
mothur >
remove.seqs(fasta=NorpaBac.trim.contigs.good.unique.good.filter.unique.precluster.fasta, accnos=NorpaBac.trim.contigs.good.unique.good.filter.unique.precluster.denovo.vsearch.accnos)
[WARNING]: This command can take a namefile and you did not provide one. The current namefile is F:\Norpatagonia\Bacteria\NorpaBac.trim.contigs.good.names which seems to match F:\Norpatagonia\Bacteria\NorpaBac.trim.contigs.good.unique.good.filter.unique.precluster.fasta.
Removed 88 sequences from your fasta file.
Output File Names:
F:\Norpatagonia\Bacteria\NorpaBac.trim.contigs.good.unique.good.filter.unique.precluster.pick.fasta
I checked that my count_table file and my fast fila have the same number of sequences but different names.
I am also attaching the mothur log-file for reference.
Thank you very much.
JuanMothur log-file