Hi guys, I used pcr.fasta command to customize the reference database: SILVA_138.1_SSURef_NR99_tax_silva_full_align_trunc.fasta. When I ran the summary.seqs command on the .pcr.fasta file, the mothur just showed “Using 16 processors” and left with blank. Anyone knows how to fix this?
Hi - can you tell me where you obtained/how you are generating that file? The file you’d get from Silva reference files would be silva.nr_v138_1.align not the file name you provided.
Pat
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