Hi, I am a french student in bio-informatics,
I try to used mothur for metagenomics analysis, I would like to know how to create a correct oligo file. I write it, so , but I don’t know how to put the “adaptator”
#Amorce 16S
forward CCTACGGGAGGCAGCAG 357F16S
reverse CCGTCAATTCMTTTRAGT 926R16S
#454MIDS
barcode ATCAGACACG PY5MID5
barcode ATATCGCGAG PY6MID6
barcode CGTGTCTCTA PY7MID7
barcode CTCGCGTGTC PY8MID8
barcode ATACGACGTA PY10MID15
#Sample
#Soulom 2008 PY5
#Peyrouse 2008 PY6
#Lagoin 2008 PY7
#Pau 2008 PY8
#Lacq 2008 PY10
#Amorces
#Echantillons Primer reverse Primer forward
primer ATCAGACACGCCGTCAATTCMTTTRAGT ATCAGACACGCCTACGGGAGGCAGCAG PY5
primer ATATCGCGAGCCGTCAATTCMTTTRAGT ATATCGCGAGCCTACGGGAGGCAGCAG PY6
primer CGTGTCTCTACCGTCAATTCMTTTRAGT CGTGTCTCTACCTACGGGAGGCAGCAG PY7
primer CTCGCGTGTCCCGTCAATTCMTTTRAGT CTCGCGTGTCCCTACGGGAGGCAGCAG PY8
primer ATACGACGTACCGTCAATTCMTTTRAGT ATACGACGTACCTACGGGAGGCAGCAG PY10
#the adaptator I want to know how to use in a oligo file
Adaptateurs
F- CGTATCGCCTCCCTCGCGCCATCAG
R- CTATGCGCCTTGCCAGCCCGCTCAG
thank you for your reply.