I am trying to recreate and study where they used modified 515f and 806r primers. I am creating the oligos files but the sequences given for a sample set are for example:
515 forward = GCCTTGCCAGCCCGCTCAGGTGTGCCAGCMGCCGCGGTAA;
806r = GCCTCCCTCGCGCCATCAGCTCGCGGGGACTACVSGGGTATCTAAT;
I did a search and found which are actually the primer sequences within these two strings, but we are clearly having adaptors and linkers in both forward and reverse. Should I list both barcodes in the file (basically two barcodes for one sample)?