make.lefse issue

Hi All
First of all, thank you for any help you can give me. I am trying to use lefse on the Huttenhower web Galaxy instance with a file that I create with the make.lefse command in mothur (latest version). I use my shared file, a design file and a constaxonomy file as inputs. Then I try to upload the lefse file as a tabular file in Galaxy but I get an error message when I use this file in the first step that is the format data for lefse option. Any help will be greatly appreciated. Thanks for mothur. It is truly great software.

Sorry, I’m not sure what they’re looking for. Could you ask Curtis’s group what they’re looking for? Also, you can run lefse in mothur using mothur-generated files.


I think i better write to them and send them the file. I did tried to figure out what the problem was but could not spot it. I did run the lefse command in mothur. I just wanted to compare the results. Thanks for your help.