Deal all

I am trying to run analysis of one single set of MiSeq 16S data on Mothur. I am using Windows 8 (64 bit exec verison of Mothur downloaded)

I have all the files in the same folder, ready to go.

I rename my forward and reverse read files to ng1.fastq and ng2.fastq

Command I input is make.contigs(ffastq=ng1.fastq, rfastq=ng2.fastq) but no luck, Mothur can’t open the files?

Can anyone advise me on this issue…soul destroying!

This may be helpful…