I was trying make contigs with 537 samples (Hiseq run 270x2). And I am getting an error at the end of the make contigs command and its not saving a outfile.
>>>>> Processing file pair Blank4_TGAGTACG-TAGCGAGT_clipped_R1.fq - Blank4_TGAGTACG-TAGCGAGT_clipped_R2.fq (files 537 of 537) <<<<< Making contigs... It took 31510 secs to process 17036298 sequences. [WARNING]: your sequence names contained ':'. I changed them to '_' to avoid problems in your downstream analysis. mothur > summary.seqs(fasta=current) [WARNING]: no file was saved for fasta parameter. You have no current fastafile and the fasta parameter is required. Using 100 processors. [ERROR]: did not complete summary.seqs.
When I tried to split this files in to two then I could able to make outfile for ONLY ONE part. Another file gave the same error.
Is it with the server side issue or with mothur?
btw, I am using mothur version 1.35.1