I have a pretty basic question. In the process of trying to use mothur to analyze my own data, I have noticed that I am able to process various subsets of my data, but if I try to analyze all my files at once, I get errors. I am pretty sure that it is because I am not using a large enough EC2 instance (i.e., the point at which I get errors is directly proportional to how many files I have attempted to process in that subset). Is there a basic guideline that could point me to how to choose the best size instance for my dataset?
Thanks so much!