After Align.sequence step, my sequence summary looks like this
I am finding it difficult to choose start-end position for screen.seqs command, since start end position is varying all over the place in the sequence data. Does this mean, sequences were not aligned properly? or having some issues with in it. how do i go about further in this scenario.
The sequence data sets were generated by V3-V4 primers.
and i am using Mothur 1.44.0 version
Many thanks in advance