I am new to mothur and I don’t really understand a lot of stuff.
Right know I am confused how I must choose parameters (start, end, min/max-lenght, homop, ambig) for screen.seq command. Do I need to find answer from my previous commands like summary.seq? Or I just need to randomly select those numbers and look what works better, then how can I know that that specific option is the best one? Or there is even some constant numbers that all bioinformations use?
Thanks in advance for answer