How to add group information to a countfile?

Commands in mothur
1

Hello,

I am trying to remove a group (sample) from my analysis.

I have tried using the commands provided in the SOP and get the following error:

mothur > remove.groups(fasta=current, count=current, groups=4508_160404_A)
Using stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.count_table as input file for the count parameter.
Using stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.fasta as input file for the fasta parameter.

[NOTE]: The count file should contain only unique names, so mothur assumes your fasta, list and taxonomy files also contain only uniques.

[ERROR]: your count file does not contain group info, aborting.


Is there a way to combined the information in my group file and count file so that I can remove all sequences and count information derived from this particular sample from my analysis?

Thanks,
Tim

2

How did you create the count file? I suspect you didn’t include a group file when running count.seqs. If you did, what happens when you run "head " from the terminal?