I have classified my reads with classify.seq() and that seems to have worked well, but when I try to pass my fasta file to dist.seq() to obtain an OTU table, I get the error “your sequences are not the same length, aborting”.
I found the same error on this forum, but there was no solution posted.
I have 16S Nanopore data, so possibly my error is caused by that?
Do I need to align the data before using dist.seq()? Because when I align it to my reference database, and then proceed with classify.seq(), the classification does not work anymore.
Luna van der Loos