[Error] before pre.clustering

Commands in mothur
1

Dear all,

I’d like to pre cluster my dataset by pre.cluster. In order to do that I need to perform
unique.seqs thereby I get an -->

[Error]: You already have a sequence named No_Name in your fasta file, sequence names must be unique, please correct.

and

[Error]: your sequences are not all the same length. pre.cluster requires sequences to be aligned.

Could someone help???

Kind regards,
Sugi

2

Mothur expects sequence names to be unique. It looks like you have more than one sequence named, “No_Name”. Can you check your fasta file?

3

Hi,

thanks for the help, I could resolve that problem with unique.seqs.

But for pre.clustering I get this Error --> “your sequences are not all the same length. pre.cluster requires sequences to be aligned”

Thanks for the help

4

Have you run align.seqs at this point in your pipeline?

5

Thanks, after aligning pre.cluster is possible…