dist.seqs requires aligned fasta file?

[yangfa1]

Hi, could you please correct me for what I guess below:

1. the input fasta file for dist.seqs must be aligned.
2. the only step in MiSeq SOP is align.seqs step. So this step cannot be skipped even if I do not want to use screen.seqs to remove any sequences.
   Thanks.

[pschloss]

Correct.