I’m going through MiSeq SOP protocol and got an ERROR message when I run count.seqs:
mothur > count.seqs(name=stability.trim.contigs.good.good.names, group=stability.contigs.good.good.groups, processors=16)
Using 16 processors.
[ERROR]: processes reported processing 1376092 sequences, but group file indicates you have 3130329 sequences. Either you have a file mismatch or a process failed to complete the task assigned to it.
I run the same command a few weeks ago using the same dataset and didn’t get any error. Now I’m going through the same steps again because I made a mistake in a previous step, but apparently this time I’m getting this error. So I don’t see how it can be due to “a file mismatch”. How could have “the process failed to complete the task assigned to it”?
Thanks in advance,