Hi, thank you for replying my question.
I’ve successfully solved the problem by following the guide from here File mismatch detected, quitting command.
the thing I did was removing the mismatched sequence from stability.trim.contig.fasta file to match the number of sequence to count_table file because the mismatched fasta are not so much only about 56 sequences.
so I run this command:
mothur > list.seqs(fasta=stability.trim.contigs.fasta)
Output File Names:
stability.trim.contigs.accnos
then I use stability.trim.contigs.accnos file to run this command below:
mothur > get.seqs(accnos=stability.trim.contigs.accnos, fasta=stability.trim.contigs.fasta)
Output File Names:
stability.trim.contigs.pick.fasta
finally i successfully run the summary.seqs using updated fasta file that match with the count table. here is the result:
Start End NBases Ambigs Polymer NumSeqs
Minimum: 1 38 38 0 3 1
2.5%-tile: 1 386 386 0 4 20747
25%-tile: 1 404 404 0 5 207461
Median: 1 405 405 0 5 414922
75%-tile: 1 419 419 0 5 622382
97.5%-tile: 1 430 430 6 8 809096
Maximum: 1 440 440 69 220 829842
Mean: 1 409 409 0 5
# of unique seqs: 829842
total # of seqs: 829842
It took 5 secs to summarize 829842 sequences.
Output File Names:
stability.trim.contigs.pick.summary
Thank you sir!