I’m trying to perform cluster.split on my sequences (3M unique sequences) as described in the MiSeq SOP (but at the genus level).
And I’m facing the following issues:
First, I got an error message stating : “splitmethod is not a valid parameter, ignoring”. I didn’t find any records of this issue anywhere
Then, despite the fact I specified the number of processors I would like to use, it doesn’t seems like there are any parallel work. Only a single counttemp and dist file is produced at the same time. Is it normal ?
I used this command in another project without facing these issues so I’m worried something is wrong here. Could you help me ?
Thanks a lot,
(By the way, I’m using Mothur 1.46.1)