Hi everyone,
I am running Mother 1.39.1
When I run the cluster.split command as shown in the MiSeq SOP
mothur > cluster.split(fasta=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.pick.fasta, count=stability.trim.contigs.good.unique.good.filter.unique.precluster.denovo.vsearch.pick.pick.pick.count_table, taxonomy=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pds.wang.pick.pick.taxonomy, splitmethod=classify, taxlevel=4, cutoff=0.03)
I get the following error:
NOTE]: Default clustering method has changed to opti. To use average neighbor, set method=average.
[ERROR]: did not complete cluster.split.
I looked at the cluster.split pages and there seem to be many options but I’m confused about which command I should run, any help would be swell?