cluster - "not in your count table"

Commands in mothur
1

Hello there,

I am currently trying to run the cluster command, and the following error is generated:

“MV is not in your count file - please correct”

I enter the following command:

cluster(column=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.dist, count=stability.trim.contigs.good.unique.good.filter.unique.precluster.uchime.pick.pick.count_table)

I was wondering if you could possibly assist with this error?

Many thanks,

Jo

2

As an update to this, I re-ran the dist.seqs command with 1 processor (as recommended in previous forum posts).

However, I get a similar error on running the cluster command:

cluster(column=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.dist, count=stability.trim.contigs.good.unique.good.filter.unique.precluster.uchime.pick.pick.count_table)

********************#****#****#****#****#****#****#****#****#****#****#
Reading matrix:     |||||||||||||||||||||||||||||||||||||||||||||||||||||||[ERROR]: MVM-RI-I124161_148_000000000-AK3MR_1_1105_21438_20444 is not in your count table. Please correct.

Any advice on this error would be much appreciated.

Many thanks,

Jo

3

Have you confirmed that the sequence is in the count table?

4

Yes, the sequence is in both the count table and the fasta file.