I am a newbie so I apologize in advance.
I am working with MiSeq sequences that are 16S V8 region
To process my sequences I follow the MiSeq SOP tutorial and I ran the commands which stated.
In the step Preparing for analysis i used
mothur > dist.seqs(fasta=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.fasta, cutoff=0.20)
I got almost 2 TB._then i ran
mothur > cluster(column=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.dist, count=stability.trim.contigs.good.unique.good.filter.unique.precluster.uchime.pick.pick.count_table)
I am getting this error
M00319_51_000000000-A9M9F_1M00319_51_000000000-A9M9F_1_1107_7720_10263 is not in your count table. Please correct.
and its stopped.
What should i do now.
Thanks in advance,