mothur

Classift.seqs error

Hi, I am having problem with my classify.seqs:

mothur > classify.seqs(fasta=vsearchcentroid.fas, count=vsearchOTU.txt, reference=MIDORI_LONGEST_GB237_CO1_MOTHUR.fasta, taxonomy=MIDORI_LONGEST_GB237_CO1_MOTHUR.taxon, cutoff=80)

Using 36 processors.
Reading template taxonomy… DONE.
Reading template probabilities… DONE.
It took 139 seconds get probabilities.

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It took 5 secs to classify 63 sequences.

[ERROR]: cannot convert 99ed3356b1d8b93eb1b6f46a7bbe1f04a623afd to an integer.

Removing group: 145 because all sequences have been removed.

Removing group: 16 because all sequences have been removed.

Removing group: 316 because all sequences have been removed.

Removing group: 7 because all sequences have been removed.

The issue is caused by the count file. After the classification is completed, mothur will read the count file and generate the *tax.summary file. It looks like the count file is not in the correct format. If you send your count file to mothur.bugs@gmail.com, I can take a closer look for you.